Coding
PPK patens

Part:BBa_K3583006:Design

Designed by: Sebastian Kusnik   Group: iGEM20_Duesseldorf   (2020-10-22)


Polyphosphate kinase (PPK) from Physcomitrella patens


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2444
    Illegal XbaI site found at 1458
    Illegal XbaI site found at 1536
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2444
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2444
    Illegal BglII site found at 2184
    Illegal BamHI site found at 418
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2444
    Illegal XbaI site found at 1458
    Illegal XbaI site found at 1536
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2444
    Illegal XbaI site found at 1458
    Illegal XbaI site found at 1536
    Illegal NgoMIV site found at 115
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2691
    Illegal SapI site found at 2623
    Illegal SapI.rc site found at 523
    Illegal SapI.rc site found at 1069
    Illegal SapI.rc site found at 1278


Design Notes

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Source

https://www.ebi.ac.uk/ena/browser/view/EDQ56484 To confirm EDQ56484 check https://www.uniprot.org/uniprot/Q2MEV6


References