Coding
PPK patens
Part:BBa_K3583006:Design
Designed by: Sebastian Kusnik Group: iGEM20_Duesseldorf (2020-10-22)
Polyphosphate kinase (PPK) from Physcomitrella patens
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2444
Illegal XbaI site found at 1458
Illegal XbaI site found at 1536 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2444
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2444
Illegal BglII site found at 2184
Illegal BamHI site found at 418 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2444
Illegal XbaI site found at 1458
Illegal XbaI site found at 1536 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2444
Illegal XbaI site found at 1458
Illegal XbaI site found at 1536
Illegal NgoMIV site found at 115 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2691
Illegal SapI site found at 2623
Illegal SapI.rc site found at 523
Illegal SapI.rc site found at 1069
Illegal SapI.rc site found at 1278
Design Notes
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Source
https://www.ebi.ac.uk/ena/browser/view/EDQ56484 To confirm EDQ56484 check https://www.uniprot.org/uniprot/Q2MEV6